RS4;11 Xenograft Model Service for ALL

The RS4;11 cell line serves as a highly robust and clinically relevant in vivo platform for investigating B-cell precursor Acute lymphoblastic leukemia (ALL), particularly for the evaluation of KMT2A-rearranged malignancies. As a premier pre-clinical contract research organization, Alfa Cytology delivers an integrated, high-precision RS4;11 Xenograft Model Service, meticulously optimized to provide reproducible, audit-ready data packages that accelerate your ALL drug discovery and development pipeline.

Overview of RS4;11 Xenograft Model for ALL

The RS4;11 xenograft model is an established in vivo translational system widely utilized in hematological oncology research for the study of ALL. By transplanting human RS4;11 lymphoblastoid cells into highly permissive, immunodeficient rodent hosts, this model effectively reproduces the systemic dissemination, aggressive bone marrow infiltration, and distinct peripheral blood pathophysiological profiles characteristic of progressive human ALL.

Biologically, the RS4;11 model is highly valued for its consistent engraftment profiles and predictable disease progression, which provide investigators with a reliable experimental window for conducting complex dosing schedules and pharmacodynamics studies. This model preserves critical human lymphoid molecular features, including the expression of the KMT2A-AFF1 (MLL-AF4) fusion gene and lineage-specific surface markers that drive blast expansion. Consequently, the RS4;11 model is extensively deployed in pre-clinical screening programs to assess the anti-leukemic potency of novel small-molecule inhibitors, targeted monoclonal antibodies, and combination therapeutic regimens in a physiologically relevant in vivo setting.

Fig 1. Experimental design of the distinct ALL models with on-demand analgesic treatment. (Schreiber T, et al., 2025)

Cell Line Information: RS4;11

The RS4;11 cell line was established from the bone marrow of a patient with B-cell precursor ALL. These cells grow as suspension cultures under standard in vitro laboratory parameters, maintaining a stable phenotype that serves as a robust proxy for KMT2A-rearranged malignancy research.

Our Services

Workflow of RS4;11 Xenograft Model Construction

• Cell Culture & Quality Control: Human RS4;11 cells are expanded in vitro using certified nutrient suspension media under optimized growth parameters. STR authentication and mycoplasma clearance verification are completed prior to inoculation to ensure absolute phenotypic identity and biological purity.
• Host Selection & Acclimatization: Standardized, healthy immunodeficient mice (e.g., NSG or NOD/SCID strains to support hematological engraftment) are sourced from validated vendors. The animals undergo a dedicated acclimatization phase to stabilize baseline biological and physiological metrics.
• Precision Inoculation: A calibrated suspension of high-viability RS4;11 cells is prepared in a sterile physiological buffer. The cellular suspension is precisely inoculated in vivo into the host cohorts via tail vein intravenous routes to facilitate systemic leukemic distribution.
• Longitudinal Growth Tracking: Following inoculation, disease progression is systematically monitored using FACS to detect human CD45+ cells or specific lymphoid markers (such as CD19) in peripheral blood. Animal weight, physical clinical indicators, and systemic disease burden are documented routinely.
• Stratification & Dosing: Once peripheral leukemic chimerism reaches a predetermined, statistically optimal range, the mice are randomized into matched experimental cohorts to ensure balanced baseline systemic dimensions before the initiation of customized therapeutic dosing regimens.

Fig 2. RS4;11 Xenograft Model Construction Workflow

Case Study - RS4;11 Xenograft Model Development

A pre-clinical validation study was conducted using the RS4;11 xenograft model to evaluate the therapeutic efficacy of a novel targeted inhibitor designed for ALL. Following precision intravenous inoculation of human RS4;11 cells into immunodeficient mice, the animals exhibited steady, systemic leukemic engraftment and highly predictable disease progression across all study cohorts. Animals assigned to the active treatment group demonstrated a clear, statistically significant reduction in circulating leukemic blasts and prolonged survival times compared to the vehicle control, confirming the model's high sensitivity and predictive reliability for screening targeted ALL therapies.

Why Choose Alfa Cytology?

• Oncology Domain Expertise: Profound experience in managing diverse hematological and lymphoid lineages, providing highly reproducible in vivo translational platforms for complex leukemia research.
• Rigorous Quality Control: Meticulous cell validation and standardized operating procedures that minimize experimental variability across all project stages.
• Tailored Experimental Design: Highly flexible protocols that adapt to specific animal strain requirements, custom dosing schedules, and unique compound properties.
• High-Resolution Deliverables: Every project concludes with a detailed, audit-ready report providing comprehensive FACS metrics and robust statistical validations.

Contact us

Accelerating your ALL pipeline requires a pre-clinical partner with the technical proficiency to execute rigorous in vivo workflows flawlessly. If you are looking to advance your novel compound or require specialized pre-clinical testing using our RS4;11 platform, please reach out to us today to discuss your project requirements with our expert scientific team.

For research use only. Not intended for any clinical use.