NALM-6 Xenograft Model Service for ALL

The NALM-6 cell line serves as a highly representative and clinically relevant in vivo platform for investigating B-cell precursor Acute lymphoblastic leukemia (ALL), particularly for the assessment of novel immunotherapies and targeted agents. As a premier pre-clinical contract research organization, Alfa Cytology delivers an integrated, high-precision NALM-6 Xenograft Model Service, meticulously optimized to provide reproducible, audit-ready data packages that accelerate your ALL drug discovery and development pipeline.

Overview of NALM-6 Xenograft Model for ALL

The NALM-6 xenograft model is an established in vivo translational system widely utilized in hematological oncology research for the study of ALL. NALM-6 cells are derived from the peripheral blood of a patient with B-cell precursor ALL and carry a characteristic t(5;12) translocation. By transplanting these human cells into highly permissive, immunodeficient rodent hosts, this model effectively reproduces the systemic dissemination, aggressive bone marrow homing kinetics, and peripheral blood pathophysiological profiles characteristic of advanced human ALL.

Biologically, the NALM-6 model is highly valued for its consistent engraftment potential and the ability to model minimal residual disease (MRD) scenarios. This model preserves critical human lymphoid molecular features, including the expression of pan-B-cell surface markers (such as CD19 and CD22) and intracellular signaling pathways that drive malignant blast expansion. Consequently, the NALM-6 model is extensively deployed in pre-clinical screening programs to assess the anti-leukemic potency of novel small-molecule inhibitors, bispecific antibodies, CAR-T cell therapies, and combination therapeutic regimens in a physiologically relevant in vivo setting.

Fig 1. Illustration of the B-cell malignancy xenograft model. (Xia Z, et al., 2024)

Cell Line Information: NALM-6

The NALM-6 cell line is a classic B-cell precursor model derived from a patient with ALL. These cells grow as suspension cultures under standard in vitro laboratory parameters, maintaining a stable phenotype that serves as a robust proxy for B-cell malignancy research.

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Workflow of NALM-6 Xenograft Model Construction

• Cell Culture & Quality Control: Human NALM-6 cells are expanded in vitro using certified nutrient suspension media under optimized growth parameters. STR authentication and mycoplasma clearance verification are completed prior to inoculation to ensure absolute phenotypic identity and biological purity.
• Host Selection & Acclimatization: Standardized, healthy immunodeficient mice (e.g., NSG or NOD/SCID strains to support hematological engraftment) are sourced from validated vendors. The animals undergo a dedicated acclimatization phase to stabilize baseline biological and physiological metrics.
• Precision Inoculation: A calibrated suspension of high-viability NALM-6 cells is prepared in a sterile physiological buffer. The cellular suspension is precisely inoculated in vivo into the host cohorts via tail vein intravenous routes to facilitate systemic leukemic distribution.
• Longitudinal Growth Tracking: Following inoculation, disease progression is systematically monitored using FACS to detect human CD45+ cells or specific lymphoid markers (e.g., CD19, CD22) in peripheral blood. Animal weight, physical clinical indicators, and systemic disease burden are documented routinely.
• Stratification & Dosing: Once peripheral leukemic chimerism reaches a predetermined, statistically optimal range, the mice are randomized into matched experimental cohorts to ensure balanced baseline systemic dimensions before the initiation of customized therapeutic dosing regimens.

Fig 2. NALM-6 Xenograft Model Construction Workflow

Case Study - NALM-6 Xenograft Model Development

A pre-clinical validation study was conducted using the NALM-6 xenograft model to evaluate the therapeutic efficacy of a novel targeted inhibitor designed for ALL. Following precision intravenous inoculation of human NALM-6 cells into immunodeficient mice, the animals exhibited steady, systemic leukemic engraftment and highly predictable disease progression across all study cohorts. Animals assigned to the active treatment group demonstrated a clear, statistically significant reduction in circulating leukemic blasts and prolonged survival times compared to the vehicle control, confirming the model's high sensitivity and predictive reliability for screening targeted ALL therapies.

Why Choose Alfa Cytology?

• Oncology Domain Expertise: Profound experience in managing diverse hematological and lymphoid lineages, providing highly reproducible in vivo translational platforms for complex leukemia research.
• Rigorous Quality Control: Meticulous cell validation and standardized operating procedures that minimize experimental variability across all project stages.
• Tailored Experimental Design: Highly flexible protocols that adapt to specific animal strain requirements, custom dosing schedules, and unique compound properties.
• High-Resolution Deliverables: Every project concludes with a detailed, audit-ready report providing comprehensive FACS metrics and robust statistical validations.

Contact us

Accelerating your ALL pipeline requires a pre-clinical partner with the technical proficiency to execute rigorous in vivo workflows flawlessly. If you are looking to advance your novel compound or require specialized pre-clinical testing using our NALM-6 platform, please reach out to us today to discuss your project requirements with our expert scientific team.

For research use only. Not intended for any clinical use.