Hs 746T Xenograft Model Service for Gastric Cancer

The Hs 746T xenograft model is a robust, MET-amplified gastric cancer preclinical platform widely used to evaluate targeted therapeutics, combination regimens, and novel drug candidates in vivo. Alfa Cytology provides fully customizable Hs 746T xenograft model services, from tumor implantation and in-life monitoring to endpoint analysis, ensuring high-quality, reproducible data to accelerate your preclinical oncology pipeline.

Overview of Hs 746T Xenograft Model for Gastric Cancer

The Hs 746T cell line was originally isolated from a patient with gastric adenocarcinoma and is characterized by high-level MET gene amplification, rendering it a highly relevant model for studying MET-driven tumor biology. In preclinical settings, Hs 746T xenografts exhibit rapid, aggressive tumor growth and robust MET signaling, making them an ideal system for evaluating MET inhibitors, multi-kinase blockers, and combination strategies targeting downstream PI3K/AKT and RAS/MAPK pathways. This model has been extensively validated in pharmacology studies and consistently demonstrates dose-dependent tumor regression upon treatment with MET-targeted agents.

Subcutaneous implantation of Hs 746T cells into immunodeficient mice produces well-established tumors with histological features consistent with human gastric adenocarcinoma, including glandular structures and high proliferative indices. The model supports both standard subcutaneous and orthotopic implantation approaches, enabling flexible study designs tailored to specific research objectives, whether assessing primary tumor growth inhibition, metastatic potential, or biomarker modulation.

Figure 1. Steady-state levels of gastric cancer cell lines. (Koustas, Evangelos, et al., 2020)

Cell Line Information: Hs 746T

Hs 746T is a human gastric adenocarcinoma cell line with well-documented molecular and phenotypic characteristics. The following table summarizes key cell line attributes relevant to preclinical xenograft studies.

Our Services

At Alfa Cytology, we offer end-to-end Hs 746T xenograft model services designed to meet the rigorous demands of preclinical oncology research. Our experienced team manages every phase of the study---from cell line authentication and tumor implantation to in-life monitoring, necropsy, and comprehensive endpoint analysis---delivering reliable, audit-ready data to support your IND-enabling and publication goals.

Workflow of Hs 746T Xenograft Model Construction

The construction of Hs 746T xenograft models follows a standardized, quality-controlled workflow that ensures tumor engraftment consistency and reproducible pharmacological readouts. Each step is executed under strict compliance with animal welfare guidelines and study-specific protocols.

1. Cell Preparation and Quality Control: Hs 746T cells are expanded under optimal culture conditions and subjected to mycoplasma testing and STR authentication to confirm identity and viability prior to implantation.
2. Cell Harvesting and Suspension: Cells are harvested at logarithmic growth phase, washed, and resuspended in a serum-free medium or Matrigel mixture at the appropriate concentration (typically 5 x 10^6 cells per 100-200 microL).
3. Animal Preparation and Implantation: Immunodeficient mice (e.g., BALB/c nude or NSG) are acclimatized, and Hs 746T cells are implanted subcutaneously into the flank using a sterile syringe. Tumor growth is monitored from Day 0.
4. Tumor Monitoring and Randomization: Tumor volumes are measured by caliper twice weekly. Once tumors reach the target volume (typically 100-200 mm^3), animals are randomized into treatment and control groups.
5. Treatment Administration and In-Life Observations: Test compounds are administered via the designated route (oral, intravenous, intraperitoneal, etc.). Body weight, tumor dimensions, and clinical signs are recorded throughout the study period.
6. Endpoint Analysis and Necropsy: At study termination, tumors are excised, weighed, and processed for histopathology, immunohistochemistry, biomarker analysis, or molecular profiling as specified in the study protocol.

Figure 2: Schematic workflow illustrating the derivation and construction of the Hs 746T Xenograft Model at Alfa Cytology.

Case Study-Hs 746T Xenograft Model Development

In a representative preclinical study, Hs 746T cells were implanted subcutaneously into immunodeficient mice to evaluate the efficacy of a novel MET-targeted therapeutic candidate. Tumors were established successfully with consistent growth kinetics, and treated animals showed dose-dependent tumor growth inhibition compared to vehicle controls. Pharmacodynamic analysis confirmed robust modulation of MET phosphorylation and downstream signaling markers in tumor tissue, supporting the model's predictive value for MET-driven gastric cancer therapeutics. (Detailed quantitative data and statistical analysis are available upon request and can be customized to meet specific study requirements.)

Why Choose Alfa Cytology?

Alfa Cytology is a trusted preclinical CRO partner with deep expertise in oncology model development and a commitment to delivering high-quality, reproducible data. Our Hs 746T xenograft service is built on rigorous scientific standards and operational excellence.

• Extensive experience with MET-amplified gastric cancer models, ensuring reliable tumor engraftment and pharmacological responsiveness.
• Comprehensive quality control, including STR authentication, mycoplasma testing, and standardized husbandry conditions.
• Flexible study designs supporting subcutaneous, orthotopic, and metastatic model configurations tailored to your research objectives.
• Integrated endpoint analysis encompassing histopathology, immunohistochemistry, biomarker quantification, and PK/PD correlation.
• Dedicated project management with transparent communication, timely reporting, and full regulatory compliance for IND-enabling studies.

Contact Us

Ready to advance your gastric cancer preclinical program with the Hs 746T xenograft model? Please reach out to us today via our inquiry form or email to learn more about our Hs 746T Xenograft Model services.

For research use only. Not intended for any clinical use.