Comprehensive Orthotopic Mouse Model Services

Orthotopic Mouse Models represent the gold standard in preclinical tumor research. By engrafting tumor cells directly into the corresponding anatomical site of origin, these models faithfully replicate the Tumor Microenvironment (TME) and organ-specific disease progression. Alfa Cytology offers a dual-platform approach to orthotopic modeling, integrating both Xenograft and Syngeneic systems. Our services provide the high-fidelity data required to evaluate drug efficacy, metastatic potential, and immune system interactions across all major and rare cancer indications.

Dual-Platform Excellence in Orthotopic Modeling

To meet the diverse needs of oncology and immunotherapy R&D, we provide specialized orthotopic construction across two primary biological platforms:

Orthotopic Xenograft Models (CDX/PDOX)

By engrafting human tumor cell lines or patient-derived tissues into immunodeficient mice at the primary organ site, we provide the premier platform for:

• Human-Specific Target Validation: Testing therapies designed for human-specific mutations or proteins.
• Precision Efficacy Profiling: Achieving accurate PK/PD correlations in a site-specific environment.
• PDOX Translation: Preserving the genetic heterogeneity of patient tumors for personalized medicine studies.

Orthotopic Syngeneic Models

By engrafting mouse-derived tumor cells into immunocompetent mice of the matching genetic strain, we create the ideal environment for Immuno-Oncology (I-O) research:

• Functional Immune Interaction: Studying the interplay between the host immune system and the tumor within its natural niche.
• I-O Therapy Evaluation: Assessing the efficacy of checkpoint inhibitors, vaccines, and cell therapies.
• TME Modulation: Investigating how the organ-specific microenvironment influences immune cell infiltration and suppression.

Fig 1. Body weights over seven weeks of orthotopic tumor growth. (Greenlee JD, et al., 2022)

Precision Methodology for Universal Orthotopic Construction

At Alfa Cytology, our construction methodology is engineered for universal applicability across all oncology indications. We combine state-of-the-art microsurgical techniques with rigorous quality control to ensure that every model—regardless of the cancer type—faithfully recapitulates the complex human tumor microenvironment.

Diverse Inoculum Expertise

We offer flexible engraftment options using either Patient-Derived Xenografts (PDOX) to preserve genetic heterogeneity or validated Cell Line-Derived Xenografts (CDX) for high-throughput consistency.

Site-Specific Microsurgery

Our specialized surgical team performs high-precision engraftment across all anatomical sites—including intracranial, intrapulmonary, intrapancreatic, and intraprostatic—ensuring localized tumor growth with minimal surgical trauma.

Advanced Longitudinal Imaging

Integration of multi-modal imaging (Bioluminescence, Fluorescence, and NIR) allows for non-invasive, real-time tracking of primary tumor kinetics and spontaneous metastatic spread.

Standardized Regulatory Compliance

Every model is constructed under strict AAALAC guidelines, ensuring that the resulting efficacy and safety data meet the rigorous documentation standards required for global IND submissions.

Our Services

Extensive Library of Validated Orthotopic Models

Alfa Cytology maintains a vast biobank of validated orthotopic models across all major and rare cancer indications. Our repository integrates both Xenograft (CDX/PDOX) and Syngeneic platforms, each characterized by consistent growth kinetics, genomic profiles, and standard-of-care (SoC) responsiveness. These ready-to-use models are optimized to provide highly reproducible, IND-ready data for your drug discovery programs.

Browse our comprehensive model list below:

Why Choose Alfa Cytology for Orthotopic Model Development?

Contact Us for Tailored Orthotopic Solutions

Alfa Cytology's orthotopic mouse model services provide a versatile and clinically relevant platform to accelerate your drug discovery pipeline. Whether you require Xenograft models for targeted efficacy or Syngeneic models for complex immunotherapies, we partner with you to generate reliable, translatable data. Contact us today to discuss how our full-spectrum orthotopic solutions can be tailored to meet your specific research milestones and accelerate your path to the clinic.

For research use only. Not intended for any clinical use.