Kinase Engineered Ba/F3 Cell Line Services

The Ba/F3 cell line is a well-established in vitro model system originally derived from murine pro-B cells. Its unique cytokine-dependent growth behavior and high genetic malleability make it an ideal platform for studying kinase-driven oncogenesis and drug resistance mechanisms. At Alfa Cytology, we provide a comprehensive library of engineered Ba/F3 cell lines, covering clinically relevant kinase mutations across multiple cancer types. Our services include custom model development, functional validation, and scalable drug screening solutions to accelerate your kinase-targeted drug discovery pipeline.

Introduction to Ba/F3 Cell Line

The Ba/F3 cell line, originally established as an interleukin-3 (IL-3)-dependent murine pro-B cell line, serves as a pivotal model system in oncology research due to its unique dependency on cytokine signaling for survival. This IL-3 dependence enables the creation of a conditional system to investigate oncogene addiction: in the absence of cytokine support, Ba/F3 cells undergo programmed cell death unless viability is maintained through the ectopic expression of constitutively active oncogenic kinases. By engineering Ba/F3 cells to express specific kinase mutations, researchers can bypass cytokine dependency and induce cytokine-independent growth-a hallmark of oncogenic transformation observed in human cancers. This genetic malleability allows precise replication of driver mutations linked to tumorigenesis, enabling mechanistic studies of kinase activation, drug resistance, and signaling pathway dysregulation. The model's simplicity and uniformity further facilitate high-throughput drug screening, offering a reductionist approach to isolate kinase-specific effects from complex tumor microenvironment interactions.

Fig. 1 Ba/F3 model and lung cancer cell lines as tools for mechanistic analysis of resistance to molecular targeted drugs. (Koga, T., et al., 2022)

Comparison of Ba/F3 Cell Model and Traditional Cell Line Model

Our Services

Ba/F3 engineered cell lines are a gold-standard model for studying kinase-driven cancers and screening targeted therapies. By integrating advanced gene editing technologies, Alfa Cytology develops a customizable library of Ba/F3 cell lines expressing specific kinase mutations (e.g., EGFR, ALK, ROS1) to replicate oncogenic signaling pathways. These models enable high-throughput drug screening, resistance mechanism studies, and biomarker validation, providing a scalable solution for precision oncology.

Our Services

As a leader in the field, we not only offer Ba/F3 kinase engineered cell line construction services but can also assist you in constructing Ba/F3 cell line-derived xenograft ( CDX) models, providing you with comprehensive and high-quality drug development services.

Common Kinase Engineered Ba/F3 Cell Lines

Below is a list of Ba/F3 engineered cell lines constructed by our company for different cancer-related kinase targets. These cell lines are widely used in basic research, drug screening and development. Whether you are screening for kinase inhibitors or exploring cancer signaling pathways, we provide you with efficient and flexible cell models to facilitate your research progress.

Note: To find out more about our cancer kinase-target engineered Ba/F3 cell lines, please inquiry our service.

Alfa Cytology empowers your kinase-targeted oncology research with our kinase-target engineered Ba/F3 cell lines-a precision tool designed to accelerate drug discovery and therapeutic optimization. Whether you need pre-engineered models for high-throughput screening or custom mutations tailored to your study, our end-to-end solutions ensure genetic stability, functional consistency, and scalability. Contact us to access our Ba/F3 Kinase Library or discuss custom solutions.

For research use only.