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The development of 2D cell lines and panels for ovarian cancer involves creating and utilizing 2D cultures of ovarian cancer cells to gain insights into the disease's behavior, genetic profile, and response to various treatments. Alfa Cytology has meticulously developed a diverse array of ovarian cancer cell lines, facilitating a wide range of experiments and comprehensive research.
Creating 2D cell lines and panels for ovarian cancer involves culturing monolayer ovarian cancer cells to study the disease's molecular mechanisms. These diverse cell lines allow researchers to investigate different ovarian cancer subtypes, analyze genetic profiles, and assess responses to therapies. These tools are crucial for understanding tumor biology, screening drugs, and developing targeted treatments.
Fig. 1 The morphology of cell lines grown under 2D cell culture conditions. (ŚWIERCZEWSKA M, et al., 2023)
Alfa Cytology has developed a comprehensive series of ovarian cancer cell lines, offering a robust experimental platform for studying the intricate mechanisms of ovarian cancer and evaluating potential therapeutic approaches. These cell lines facilitate not only foundational research but also play a crucial role in drug screening and the development of personalized treatment strategies.
Sample Collection and Processing
Collection: Tumor tissue samples are obtained during surgery or biopsy under sterile conditions and sent to the lab promptly.
Processing: Tumor tissue is washed with saline or culture medium to remove blood and impurities, then cut into small pieces (1-2 mm).
Cell Isolation
Enzymatic Digestion: Cells are released from tumor tissue using collagenase, trypsin, or other enzymes, incubated at 37 ℃ until soft.
Mechanical Dispersion: Cells are further dispersed by gentle shaking or using a cell sieve to obtain a single cell suspension.
Cell Culture
Medium Selection: Choose the appropriate medium based on cell type, such as DMEM or RPMI-1640, and supplement with necessary growth factors and antibiotics.
Inoculation: Seed the isolated cells into culture flasks or plates, ensuring appropriate density to facilitate cell attachment and growth.
Cell Line Characterization and Analysis
Genomic and Transcriptomic Analysis: Techniques such as genome sequencing and RNA sequencing are utilized to understand the molecular characteristics of the cell lines.
Proteomics: Protein expression and modification statuses are analyzed to provide a comprehensive understanding of cellular functions.
Functional Testing
Drug Screening: The sensitivity of cell lines to various anticancer drugs or therapeutic agents is assessed to identify potential treatment options.
Biomarker Characterization: Potential biomarkers for diagnostic or therapeutic use are screened and validated.
Our research team offers a wide array of ovarian cancer cell lines for research and drug development.
Human-Derived Cell Lines
Mouse-Derived Cell Lines
Case Study
Two-dimensional (2D) cell culture serves as the foundational platform for our primary drug activity screening and mechanistic investigations. In this study, we performed comparative antitumor efficacy assessments of the candidate compounds REL01 and REL02 across a panel of human ovarian cancer cell lines.
To align with the compounds' targeted mechanisms of action, we selected three distinct ovarian cancer cell lines—OVCAR-3, TOV112D, and SK-OV-3—for parallelized sensitivity testing.
OVCAR-3
TOV112D
SK-OV-3
Fig. 2 Different ovarian cancer cell lines used for antitumor efficacy testing
The half-maximal inhibitory concentrations (IC50) of REL01 and REL02 were determined using standardized cell viability assays (CCK-8). These data were utilized to quantify the potency and assess the broad-spectrum antiproliferative activity of the candidate compounds across the selected models (Fig. 3).
We evaluated the antitumor efficacy of Drug A in this orthotopic xenograft model of ovarian clear cell carcinoma. Representative histological analysis is shown in Figure 3. Compared to the vehicle control group, Drug A treatment significantly improved overall survival (Fig. 4).
Fig. 3 In vitro evaluation of the inhibitory activity of REL01 and REL02 against different ovarian cancer cell lines
With amassed extensive expertise in developing ovarian cancer cell lines, Alfa Cytology dedicated to offering high-quality, customized services. Should you have any questions about our services or research, please don't hesitate to contact us.
Reference
For research use only.
Alfa Cytology, located in New York, specializes in preclinical research solutions to support ovarian cancer therapy development, offering a streamlined process from target discovery to IND application.
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