Syngeneic Mouse Model Establishment using 4T1 Breast Cancer Cell Line
Alfa Cytology specializes in establishing syngeneic mouse models for breast cancer research, with a focus on the widely used 4T1 breast cancer cell line. This robust model faithfully replicates the tumor growth, immune microenvironment, and metastatic behavior seen in human triple-negative breast cancer, making it an invaluable tool for preclinical evaluation of novel therapeutic strategies.
Our Services
4T1 Cell Line Implantation
Orthotopic or subcutaneous inoculation of 4T1 cells into immunocompetent BALB/c mice to establish a clinically relevant tumor model.
Tumor Growth and Metastasis Monitoring
Comprehensive monitoring of primary tumor progression and spontaneous metastasis, particularly to lungs, liver, and lymph nodes.
Key Advantages
| Immunocompetent Microenvironment | Our syngeneic models preserve the native immune context, enabling accurate evaluation of immune-based therapies such as checkpoint inhibitors and cancer vaccines. |
| Metastatic Behavior Mimicry | 4T1 models display spontaneous metastasis patterns similar to those observed in aggressive human breast cancers, allowing for assessment of anti-metastatic agents. |
| Reproducible and High-Throughput | Well-characterized protocols ensure consistent tumor engraftment and growth, ideal for comparative studies and screening large compound libraries. |
| Customizable Experimental Design | We tailor tumor implantation sites, dosing schedules, treatment regimens, and endpoints to meet your specific research goals and project timelines. |
Download our flyer to learn how the 4T1 syngeneic mouse model can accelerate your breast cancer research. For tailored modeling services or technical consultation, please contact us today.
Case Study - Subcutaneous and Breast Pad Tumor Model
The 4T1 cells were administered to BALB/c mice at 7 weeks of age via subcutaneous and in situ breast pad injections, respectively, and tumor volumes were assessed at various time points.
Fig.1 The Tumor Growth Curves of 4T1 Cancer Syngeneic Model.
Fig.2 Body Weight Change Curve of 4T1 Cancer Syngeneic Model.
The 4T1 model in BALB/c mice was characterized by its ease of replication. Tumor volume was anticipated to reach 100-200mm3 on the 8th day post-inoculation, followed by a subsequent increase to 2000mm3 on the 22nd day post-inoculation (Fig.1 and Fig.2). The tumor growth curve in the 4T1 model remained stable, indicating a facile tumor formation process.
