Breast Cancer Organoid Modeling Services
Breast cancer organoid modeling creates 3D cultures of tumor cells that closely mimic actual breast tumors. These organoids provide a more physiologically relevant model than traditional 2D cultures, allowing researchers to study tumor behavior, drug responses, and the tumor microenvironment. Alfa Cytology offers a comprehensive suite of breast cancer organoid modeling services tailored for research and clinical applications.
Introduction of Breast Cancer Organoid Models
Breast cancer (BC) is the most common malignant tumor in women, and its incidence rate ranks first among female malignant tumors. Tumor organoids are the concept of combining organoids and tumors, that is, tumor cells form a microstructure similar to human tumors under appropriate culture conditions and highly restore the tissue structure and gene lineage of human tumors, which can maintain tumor heterogeneity. The construction of BC organoids is mainly composed of three elements, namely BC cells, extracellular matrix, and cytokines. BC organoids can not only promote the personalized treatment of cancer patients but also can be used for the screening of new tumor drugs and the study of the process and mechanism of BC development, etc., which has profound and revolutionary significance for the research of BC.
Fig.1 An organoid culture protocol for ex vivo expansion of mammary stem cells isolated from mouse tissue. (Cui J, et. al., 2021)
Our Services
Alfa Cytology can select appropriate culture conditions and materials according to your needs quickly to establish BC organoid models. Our goal is to facilitate the robust and reproducible growth of organoids. From basement membrane extracts to growth factors and small molecules, we can create ideal organoid culture conditions that reduce experimental variability. The BC organoid model has the characteristics of convenient material collection, short culture cycle, and genetic stability, which can restore the tissue and molecular characteristics of BC cells in vivo to the greatest extent, and reproduce part of the mammary gland function. BC organoid models have both the advantages of genetic manipulation of tumor cell lines and the three-dimensional complex system characteristics of mouse human-derived tumor tissue xenograft models. Therefore, BC organoids have broad application prospects in the occurrence and development of BC, metastasis, and drug resistance mechanisms, related drug screening, individualized treatment, and transformation of research results.
Fig.2 Culture protocol for breast organoids.
Advantages of BC Organoid Models
- Low cost and short cultivation period
- Genome stability was maintained after multiple passages
- Highly preserved biological characteristics and heterogeneity of orthotopic tumor tissue
- Organoids can be used to create BC organoid-derived xenografts
- Artificial control more realistic reproduction of BC tumor tissue structure
Application Fields
- Biological characteristics of BC tumor cells
- Assess drug efficacy
- BC tumor pathogenesis and metastasis pathway
- Large-scale drug screening
Case Study
CDK4/6 Inhibitor-Resistant Breast Cancer Organoid Model
- Model Introduction
A clinically relevant in vitro platform for studying CDK4/6 inhibitor resistance mechanisms and screening novel therapeutic strategies for treatment-resistant breast cancer.
- Model Information
- Model: CDK4/6 Inhibitor-Resistant Breast Cancer Organoid Model
- Resistance Profile: CDK4/6 Inhibitor-Resistant
- Cancer Type: Invasive Ductal Carcinoma (IDC)
- Culture System: 3D Organoid Culture
- Model Construction
The 3D organoid model was established from CDK4/6 inhibitor-resistant breast cancer cells cultured under optimized conditions. Through precise seeding density control and specialized culture protocols, the model maintains stable resistance characteristics and tumor heterogeneity, enabling reproducible drug testing and resistance mechanism investigation.
Fig. 3 Construction workflow of CDK4/6 inhibitor-resistant breast cancer organoid model. (Source: Alfa Cytology)
- Model Data
- Stable Resistance Phenotype: Drug sensitivity testing confirmed significant resistance to the CDK4/6 inhibitor, demonstrating high cell viability under drug treatment and substantially elevated IC₅₀ values compared to sensitive controls.
- Sustained Proliferative Capacity: The resistant organoids maintained robust proliferative capacity despite continuous drug exposure, providing a reliable platform for evaluating combination therapies and overcoming resistance mechanisms.
Fig. 4 Histological morphology and drug sensitivity of CDK4/6 inhibitor-resistant breast cancer organoid model. (Source: Alfa Cytology)
Alfa Cytology has many years of experience in constructing BC organoid models. Based on our advanced platform and experienced scientists, we can build customized, cost-effective, high-quality BC organoids to assist you in your BC organoid model research. If you are interested in learning more about our BC organoid services and solutions please contact us. We will give you a professional and competitively priced strategy that suits your needs as quickly as possible.
Reference
- Cui J, Guo W. Establishment and long-term culture of mouse mammary stem cell organoids and breast tumor organoids. STAR Protoc. 2021 May 29;2(2):100577.