Thyroid Cancer Zebrafish Models

Introduction to Zebrafish Models

Zebrafish (Danio rerio) are vital in thyroid cancer research due to their rapid development, transparent embryos, and ease of genetic manipulation. These features allow for high-throughput drug screening and real-time observation of tumor growth. With similarities to human thyroid structure and function, zebrafish offer a cost-effective alternative to mammalian models, making them essential for drug discovery and tumor biology studies in thyroid cancer.

Characteristics of Thyroid Cancer Zebrafish Models

• Rapid Development: Zebrafish embryos develop within 24 hours, allowing for quick experimental timelines in thyroid cancer research.
• Transparency: The transparent nature of zebrafish embryos permits direct observation of tumor growth, angiogenesis, and metastasis without invasive procedures.
• Genetic Manipulability: Techniques enable easy creation of transgenic and knockout models, facilitating the study of specific genetic alterations in thyroid cancer.
• Physiological Relevance: Zebrafish have a thyroid structure and hormonal regulation that closely resemble those of humans, providing valuable insights into cancer biology.
• Cost-effectiveness: Maintaining zebrafish is significantly cheaper than traditional mammalian models, making them accessible for high-throughput screening and research.
• Dynamic Imaging Capabilities: Advanced imaging techniques allow real-time tracking of tumor progression and the effects of therapeutic interventions.

Our Services

The creation of thyroid cancer zebrafish models typically involves injecting thyroid cancer cell lines into the yolk sac or body cavity of zebrafish embryos. This process allows for precise monitoring of tumor growth and behavior in a live organism. Researchers can utilize established cell lines or primary tumor samples for transplantation, enabling the exploration of various therapeutic responses and tumor dynamics in a highly efficient and observable environment.

Gene Editing Model Development

• Utilize CRISPR/Cas9 for precise introduction of thyroid cancer mutations (BRAF^V600E, RET/PTC, RAS).
• Dual knockout of TP53 and PTEN to mimic advanced cancer features.
• Addresses molecular mechanisms of PTC, FTC, and MTC, and explores MAPK/PI3K interactions.

Patient-Derived Xenografts (PDX)

• In situ transplantation of patient tumors into zebrafish embryos or adults for heterogeneity retention.
• Live fluorescent tracking of metastasis.
• Enables personalized drug sensitivity testing and studies resistance mechanisms.

Chemical-Genetic Induction Models

• MNNG exposure with TSH stimulation to simulate tumorigenesis.
• Explores environmental and genetic interactions in a BRAF-mutant background.

Our Advantages

Speed Gene editing models delivered in 8 weeks—six times faster than mouse models. PDX efficacy data ready in 15 days.	Cost Models at 20% of the cost of mammalian models, with tiered pricing options (Basic, Standard, Custom).
Validation Clinically validated with TCGA data to ensure alignment with patient profiles.	Translational Support GLP-compliant data packages for smooth transition from animal studies to FDA submission.

Alfa Cytology is your ideal partner for developing thyroid cancer zebrafish models, supported by our extensive expertise in oncology research. We provide ready-to-use models, customizable options, and collaborative R&D services tailored to your specific needs. From solutions to detailed reports, we offer comprehensive support throughout your research journey. Contact us for inquiries or to discuss custom model development.

For research use only.