CML Models Development Services
To decipher CML molecular pathology and advance therapeutic development, Alfa Cytology engineers precision chronic myeloid leukemia (CML) animal models leveraging CRISPR/Cas9 gene editing. Our BCR-ABL1-validated platforms enable mechanistic studies of disease progression and drug resistance, with high engraftment success ensuring experimental rigor.
Introduction to CML Models
CML is a chronic myeloproliferative tumor that accounts for about 15% of adult-diagnosed leukemia cases. The CML model is helpful in revealing the pathogenesis of CML, the mechanism of drug resistance and the development of new therapies. A variety of in vivo models have been established so far to develop and improve treatments for the molecular mechanism of cml. These models include transgenic model, xenotransplantation model and humanized model. With the development of immunodeficient xenotransplantation models, it has become possible to use cells from CML patients for in vivo research.
Fig. 1. CML human and murine models till date. (Sontakke, P. et al., 2016)
To reveal the molecular pathology of chronic myeloid leukemia and to develop and improve therapies, Alfa Cytology provides various animal model development services for CML, including but not limited to the following.
| Conventional Transgenic Models of CML | BCR-ABL1 knock-in models via pronuclear microinjection, exhibiting chronic-phase myeloproliferation. Integration sites confirmed by fluorescence in situ hybridization (FISH). |
| Conditional Transgenic CML Models | Tetracycline-responsive systems (Tet-On) enabling time-controlled BCR-ABL1 expression and staged leukemogenesis. |
| Retroviral Transduction Models of CML | BCR-ABL1 delivery into murine hematopoietic progenitors using replication-incompetent retroviral vectors, generating accelerated-phase phenotypes. |
| Xenograft Models of CML | Engraftment of clinically annotated CML samples (chronic/blast crisis phases) in immunocompromised strains, preserving tumor heterogeneity with serial peripheral blood monitoring. |
| Humanized Mice Models of CML | Co-engraftment of CD34⁺ human hematopoietic stem cells with primary leukemic blasts to reconstruct patient-specific bone marrow niches for immunotherapy evaluation. |
Advantages of Our Services
- We specialize in providing services for customizing CML models according to your specific research needs.
- With our extensive experience, we excel at constructing a wide range of CML models, ensuring flexibility and diversity in your research options.
- Our services include providing a model with a CML phenotype, guaranteeing the rigor and relevance of your experiments.
Alfa Cytology offers comprehensive CML animal model services aimed at investigating the interplay between leukemic cells and their microenvironment, as well as the pathogenesis of CML. Our services accelerate the study of the molecular mechanisms underlying CML pathogenesis and facilitate the development of novel targeted therapies. Please contact us if you have any questions or need more information about our services.
Reference
- Sontakke, P.; et al. Modeling of Chronic Myeloid Leukemia: An Overview of In Vivo Murine and Human Xenograft Models. Stem Cells Int. 2016, 2016: 1625015.