ALL Models Development Services

To address acute lymphoblastic leukemia (ALL) heterogeneity, Alfa Cytology engineers genetically defined ALL models recapitulating pediatric/adult subtypes. Our CRISPR-validated system enables targeted studies of NOTCH1 mutations, cytokine microenvironments, and CNS infiltration mechanisms.

Introduction to ALL Models

ALL is a prevalent condition among children, characterized by abnormal proliferation of lymphoblasts. With the advent of new drugs and treatments, the overall cure rate for ALL has reached close to 90%. The utilization of ALL models plays a crucial role in drug development, as it enables the simulation of the human tumor microenvironment, evaluation of drug efficacy, and investigation of the underlying pathogenesis. Various ALL system models, including syngeneic and xenograft models, are employed for research purposes, with mice being commonly chosen as model organisms due to their genetic and hematopoietic similarities to humans. The construction of ALL animal models holds great promise in advancing the development and treatment of leukemia.

Fig. 1. Major strategies for establishing animal models of ALL. (Xin, Q. et al., 2022)

Animal models provide us with an excellent tool to decipher complex ALL pathogenesis, evaluate novel targeted therapies in vivo, and assess potential drug toxicities. Alfa Cytology delivers specialized ALL model development services including:

Spontaneous ALL Animal Models

While spontaneous ALL models are rare, we leverage AKR/J mice with endogenous retrovirus-driven lymphomagenesis. These models exhibit CD3⁺/CD19⁻ immunophenotypes characteristic of spontaneous T-cell leukemia, enabling studies on early leukemogenic events.

Induced ALL Animal Models

Transgenic ALL Animal Models

Through the utilization of CRISPR-Cas9 technology, we specialize in the establishment of transgenic animal models. These models enable us to identify specific drug targets for leukemia and enhance treatment strategies for the disease. Our services encompass a range of ALL model builds, including but not limited to the following:

Xenograft Models of ALL

We offer xenograft model construction services by utilizing cells or tissues obtained from patients with ALL. Through the transplantation of B or T-cell ALL cells from these patients into immunodeficient animals, we induce the development of ALL and generate reliable ALL animal models to support research and experimentation.

Customization of ALL Models in Other Species

We provide a comprehensive selection of animal models for ALL, encompassing mice, rats, zebrafish, fruit flies, and guinea pigs. These diverse animal models can be utilized to meet the specific research needs and objectives of our clients.

Advantages of Our Services

• With our extensive experience in constructing ALL models, we boast an exceptionally high success rate.
• We have the capability to provide you with the specific ALL subtype animal model you desire.
• We are proficient in building ALL models across a diverse range of animal species, ensuring flexibility and suitability for your research needs.

Alfa Cytology provides ALL animal model design, construction, and screening services. Our goal is to provide customers with professional, high-quality ALL animal models. The animal models we provide will help you develop new therapeutic drugs to support the study of your pathogenesis. Please contact us if you need more information about our services in ALL animal models.