T-cell Engineering

T-cell Engineering involves the precise introduction of ETES modules into endogenous T cells, equipping them to specifically target tumor cells without compromising physiological immune function. At Alfa Cytology, we specialize in genetically modifying T-cells to enhance their immune activation, ensuring that they can effectively engage with tumor cells and provide long-lasting anti-tumor responses.

Introduction to T-cell Engineering

ETES technology leverages endogenous T-cell signaling pathways. Engineered T cells expressing ETES modules maintain native receptor signaling mechanisms, promoting robust immune activation strictly upon tumor antigen recognition. This methodology significantly enhances T-cell specificity, safety, and persistence compared to conventional synthetic receptors.

Fig.1 T cell functionality and longevity. (Kishton, R. J., et al., 2017)

Types of T-cell Engineering

• Autologous T-cell Engineering: T-cells are extracted from the patient's own blood, genetically modified to target tumor antigens, and then reinfused. This approach reduces the risk of immune rejection.
• Allogeneic T-cell Engineering: T-cells from healthy donors are genetically modified and expanded for use in patients with cancer, ideal for patients who cannot provide their own T-cells.
• Off-the-Shelf T-cells: Pre-engineered T-cells are stored and ready for use in clinical settings, allowing faster therapeutic intervention.

Our Services

In the ETES™ Platform, T-cell engineering is seamlessly integrated to optimize the natural immune response. The platform efficiently isolates and expands T-cells, then introduces ETES™ modules to enhance targeting and activation. This ensures that the engineered T-cells are functional, capable of recognizing tumor antigens, and primed for preclinical testing.

Selection of Optimal T-cell Populations

Specific T-cell subsets are identified and enriched based on their proliferation potential, functional robustness, and suitability for ETES module integration.

ETES Module Integration

State-of-the-art genetic manipulation technologies—including viral transduction and electroporation—are employed to integrate ETES modules into selected T-cell populations with high efficiency, stability, and expression consistency.

Phenotypic and Functional Validation

Engineered T cells undergo comprehensive in vitro assays, such as flow cytometry and cytokine secretion profiling, to verify successful ETES module expression, antigen-specific recognition, and functional T-cell activation.

Quality Control and Stability Analysis

Rigorous quality control assays evaluate engineered T-cell viability, purity, and genetic stability, ensuring consistency and reliability of the engineered cell product for downstream applications.

Workflow of T-cell Engineering

Contact Us

Ready to enhance your cancer research with ETES-engineered T-cells? Let Alfa Cytology help you design and optimize T-cell therapies for preclinical studies. contact us to discuss how we can support your project's success with our cutting-edge T-cell engineering services.

For research use only.