ETES^TM Platform: Harnessing Endogenous T-cell Defense Against Tumors

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Project Description

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ETES Modules Design

ETES Modules are custom-designed fusion molecules engineered to harness the body's endogenous T-cell responses without disrupting native immune signaling pathways. At Alfa Cytology, we specialize in designing and optimizing these ETES modules to ensure that T-cells can effectively target and eliminate tumor cells, enhancing cancer treatment precision and safety.

Introduction to ETES Modules Design

Unlike traditional synthetic receptors that disrupt native T-cell receptor (TCR) signaling, ETES modules maintain physiological immune processes. By integrating carefully selected antigen-binding extracellular domains with intracellular signaling motifs that mirror natural T-cell co-receptor function, ETES ensures robust yet controlled T-cell activation directly triggered by the presence of cancer-specific antigens.

The Modular Structure of ETES Molecule

Antigen Binding Domain

Engineered extracellular domains tailored specifically for recognition of distinct cancer antigens.

TCR Binding Domain

Designed for precise and effective engagement of the native T-cell receptor (TCR) complex on T-cells, ensuring potent T-cell activation.

Co-Receptor Domain

Custom intracellular domains that modulate T-cell responses to improve activation specificity and persistence.

Categories of ETES Modules

Single-Antigen ETES Modules

Designed to precisely target a specific tumor-associated antigen (TAA), these modules enable focused and highly efficient therapeutic responses, ideal for tumors characterized by consistent antigen expression.

Multi-Antigen ETES Modules

Engineered to simultaneously recognize multiple tumor-associated antigens, these modules effectively address tumor heterogeneity and prevent tumor escape due to antigen loss, improving therapeutic efficacy in more complex tumor environments.

Conditional Activation ETES Modules

These modules incorporate inducible or condition-dependent signaling motifs, activating only in the presence of specific tumor microenvironment signals. This design significantly enhances safety and specificity, reducing potential off-target effects and immune toxicity.

Affinity-Optimized ETES Modules

These ETES modules feature engineered extracellular domains with optimized binding affinities to tumor antigens, enhancing selective targeting, therapeutic potency, and reducing off-target immune activation.

Our Services

At Alfa Cytology, our approach begins with detailed profiling of your targeted tumor antigen and the surrounding immune microenvironment. Our expert bioengineering team subsequently designs ETES™ modules, optimizing both the extracellular antigen-binding domains and the intracellular activation sequences.

Antigen Profiling and Selection

Extensive tumor-specific antigen characterization is conducted using advanced bioinformatics and laboratory-based techniques. Optimal targets are identified based on their specificity, expression profiles, and potential for minimal cross-reactivity.

Computational Design and Optimization

State-of-the-art computational modeling is applied to optimize ETES™ constructs. Techniques including codon optimization, molecular dynamics simulations, and affinity maturation ensure maximal binding affinity, stability, and functionality prior to empirical synthesis.

ETES Construct Synthesis

Advanced gene editing technologies, such as CRISPR/Cas9, alongside precision synthetic biology methodologies, are employed in assembling high-quality ETES™ constructs. This step ensures optimal expression and functional integrity within engineered T-cells.

Functional Validation

Rigorous in vitro validation protocols are implemented to confirm ETES™ construct performance. Assays including binding affinity tests, T-cell activation profiling, cytokine secretion quantification, and specificity evaluations ensure comprehensive functional validation.

Workflow of ETES Modules Design

Construct Assembly

Codon-optimized ETES™ constructs are synthesized and subjected to stringent in vitro expression analysis to ensure functional integrity.

Delivery System Integration

Validated constructs are systematically integrated into carefully selected viral or non-viral delivery systems, preparing constructs for immediate T-cell engineering and preclinical applications.

Module Blueprinting

Detailed antigen profiling is conducted, informing selection of the optimal extracellular binding domains and intracellular activation motifs tailored to specific tumor targets.

Functional Testing

Each construct undergoes extensive validation through affinity assays, T-cell activation analysis, cytokine secretion profiling, and specificity tests, ensuring precise performance against targeted antigens.

Contact Us

Looking for precise and targeted ETES modules to enhance your cancer research? Let Alfa Cytology help you design tailored solutions for your preclinical studies. With our expertise in custom module development and testing, we can support your project's success every step of the way.Feel free to contact us to discuss your specific needs or explore collaboration opportunities.

For research use only.